Kolegij
Studiji
Medical Studies in EnglishStudijska godina
1ISVU ID
292660ECTS
1.5
Major goal of this course is to familiarize students with a) basic concepts and definitions in genetic engineering, b) modern genetic engineering techniques and c) their applications in medicine. The second goal is to transfer to the students knowledge and skills required for work in research laboratories in which recombinant DNA technology is routinely used. The third goal of this course is to enable students to independently form an informed opinion about recombinant DNA technology. Upon completion of this course the students will be able to understand basic principles and methodology of genetic engineering, and will be able to plan and perform a construction of a recombinant plasmid DNA.
1) Zabilješke s predavanja i vježbi
2) Odabrana poglavlja iz a) Molecular Biology of the Cell – 6th edition (2015)
Autori: Bruce Alberts, Alexander Johnson, Julian Lewis, David Morgan,
Martin Raff, Keith Roberts, Peter Walter
b) Gene cloning & DNA analysis – 7th edition (2016)
Autor: T. A: Brown
c) Molecular Cloning: A laboratory manual – 4th edition (2012)
Autori: Michael R. Green and Joseph Sambrook
1) Molecular Clonong – Technical Guide, New England Biolabs, slobodno dostupno na
https://www.neb.com/~/media/NebUs/Files/Brochures/Cloning_Tech_Guide.pdf
Pohađanje nastave i aktivno sudjelovanje u predavanjima i vježbama.
Ocjenjivanje studenata provodi se prema važećem Pravilniku o studijima Sveučilišta u Rijeci, te prema Pravilniku o ocjenjivanju studenata na Medicinskom fakultetu u Rijeci (usvojenom na Fakultetskom vijeću Medicinskog fakulteta u Rijeci). Ocjenjivanje studenata vrši se primjenom ECTS (A-E) i brojčanog sustava (1-5). Ocjenjivanje u ECTS sustavu izvodi se apsolutnom raspodjelom, te prema dodiplomskim kriterijima ocjenjivanja. Studenti tijekom nastave mogu prikupiti 70%, a na završnom ispitu 30% od konačne ocjene.
Ispitna razdoblja i prijava ispita
Prvi ispitni termin za završni test biti će odmah po završetku nastave.
Ispiti se prijavljuju u ISVU sustavu.
Ostali ispitni termini će biti navedeni u na mrežnim stranicama Centra.
Ishodi učenja
After the first lecture, students will be able to:
Understand the definition of genetic engineering and provide examples of the use of genetic engineering in real life
Understand and describe the basics of nucleic acids structure, replication, transcription, and translation
Solve basic problems related to DNA/RNA structure and processes of replication, transcription, and translation
Ishodi učenja
After the 2nd lecture, students should be able to:
Explain the terms endonuclease, exonuclease, ligase, polymerase, kinase, phosphatase, recombinase, restriction endonuclease, reverse transcriptase, sticky ends, blunt ends, palindromic sequences.
Describe the activities of DNA manipulative enzymes in general terms.
Describe and explain the principle of gel electrophoresis of DNA molecules.
Solve basic problems related to activities that DNA manipulative enzymes exhibit on various nucleic acid substrates.
Ishodi učenja
After the 3rd lecture, students will be able to:
Understand and explain the most basic types and properties of the most common vectors used for gene cloning in E. coli.
Understand and explain the differences and similarities between genomic and cDNA libraries.
Ishodi učenja
After the 4th lecture, students will be able to:
Understand and explain the basic principles of a PCR reaction, primer design, and selection of appropriate thermocycling conditions.
Understand and explain the issue of fidelity of PCR amplification with Taq DNA polymerase.
Describe and explain the principle of gel electrophoresis of PCR products.
Solve basic problems related to PCR and DNA electrophoresis of DNA molecules.
Ishodi učenja
After the 5th lecture, students will be able to:
Understand and explain the process of DNA denaturation/renaturation, and basic principles of nucleic acid hybridization analyses and their applications.
Solve basic problems related to hybridization-based nucleic acid assays.
Ishodi učenja
After the 6th lecture, students will be able to:
Understand and describe Sanger dideoxy sequencing and Illumina SBS sequencing methods.
Deduce the order of nucleotides in an unknown DNA sequence based on the results of the Sanger sequencing.
Ishodi učenja
After the 7th lecture, students will be able to:
Name and briefly describe examples of uses of genetic engineering in medicine.
Perform basic interpretation of DNA profiles obtained by DNA fingerprinting.
Understand the basic principles of CRISPR/Cas9 technology.
Ishodi učenja
By the end of the 1st laboratory exercise, students should:
Acquire basic proficiency in accurate and reproducible pipetting using air-displacement micropipettes
Be able to understand and describe the overall goal of the laboratory exercices
Ishodi učenja
By the end of the 2nd laboratory class, students will be able to independently:
Isolate a plasmid DNA from E. coli cells.
Set-up a PCR, and a restriction enzyme digestion reaction.
Prepare DNA samples for agarose gel electrophoresis.
Load DNA samples into wells of an agarose gel.
Run an agarose gel.
Understand the requirement for the use of ethidium-bromide and other DNA stains for visualizing DNA in gels.
Interpret the results of PCR and restriction enzyme digestion based on an observed band pattern on agarose gel.
Ishodi učenja
By the end of the 3rd laboratory class, students should be able to independently:
Purify any DNA fragment from agarose gel following agarose gel electrophoresis.
Estimate the integrity, purity, and quantity of isolated DNA fragment(s).
Ishodi učenja
By the end of the 4th laboratory class, students should be able to:
Independently set-up and perform a DNA ligation reaction.
Independently set-up and perform transformation of competent E. coli cells with foreign DNA.
Evenly plate bacterial cell from liquid culture onto solid agar plates.
Ishodi učenja
By the end of the 5th laboratory class, students should be able to independently:
Plan and perform gel electrophoresis of plasmid DNA isolated from obtained transformants.
Identify the putative positive clones suitable for further analysis based on the electrophoretic pattern of isolated plasmid DNA molecules.
Ishodi učenja
By the end of the 6th laboratory class, students should be able to independently:
Plan and perform restriction digestion and gel electrophoresis of digested plasmid DNA to identify the clones carrying the insert in the correct orientation
Ishodi učenja
By the end of the 7th laboratory class, students should be able to independently:
Plan and execute construction of a recombinant plasmid DNA molecule.
| Akademska godina | |
|---|---|
| 2025/2026 | Download |